Name: ALCALIPHILIC AMPHIBACILLUS STRAINS MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. 931 #

Equipment needed: Autoclave Anaerobic workbench Filter

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
KH₂PO₄	0.2	g	0.2	1.47
MgCl₂	0.1	g	0.1	1.05
NH₄Cl	0.5	g	0.5	9.348
KCl	0.2	g	0.2	2.683
Na₂CO₃	63.6	g	63.6	600.068
NaHCO₃	50.4	g	50.4	599.95
Na₂S x 9 H₂O	0.7	g	0.7	2.914
Yeast extract	0.2	g	0.2	-
Sucrose	5.0	g	5	14.607
Modified Wolin's mineral solution	1.0	ml	-	-
Modified Wolin's mineral solution	10.0	ml	-	-
Resazurin	1.0	mg	1.0e-3	0.004
Distilled water	1000.0	ml	-	-
1 Final pH 9.5-10.0
2 Prepare the medium without adding the vitamins, sucrose, yeast extract, NH₄Cl, Na₂CO₃, and NaHCO₃ using anaerobic conditions, under nitrogen. If the medium has been boiled to remove oxygen add the NH₄Cl, Na₂CO₃, and NaHCO₃ after the medium had cooled. Dispense into tubes stopper with rubber stoppers (serum tubes or bottles, or screw capped tubes). Autoclave, and to the cooled medium add the vitamins (filter sterilised under N₂), sucrose, yeast extract, and Na₂S x 9 H₂O from anaerobic, sterile stock solutions.

Modified Wolin's mineral solution #

1000

Solution occurs in 166 other media (first defined in Medium 141).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Nitrilotriacetic acid	1.50	g	1.5	7.848
MgSO₄ x 7 H₂O	3.00	g	3	12.172
MnSO₄ x H₂O	0.50	g	0.5	2.958
NaCl	1.00	g	1	17.112
FeSO₄ x 7 H₂O	0.10	g	0.1	0.36
CoSO₄ x 7 H₂O	0.18	g	0.18	1.161
CaCl₂ x 2 H₂O	0.10	g	0.1	0.68
ZnSO₄ x 7 H₂O	0.18	g	0.18	0.626
CuSO₄ x 5 H₂O	0.01	g	0.01	0.04
AlK(SO₄)₂ x 12 H₂O	0.02	g	0.02	0.042
H₃BO₃	0.01	g	0.01	0.162
Na₂MoO₄ x 2 H₂O	0.01	g	0.01	0.041
NiCl₂ x 6 H₂O	0.03	g	0.03	0.231
Na₂SeO₃ x 5 H₂O	0.30	mg	3.0e-4	0.001
Na₂WO₄ x 2 H₂O	0.40	mg	4.0e-4	0.001
Distilled water	1000.00	ml	-	-
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Last modified:	25.03.22
Source:	DSMZ
Taxonomic range:	Bacteria
Medium type:	Complex medium
Final pH:	9.5 - 10.0
Equipment needed:	Anaerobic workbench, Autoclave, Filter

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