Main sol. 931
Internal Solution-ID: S1920
Equipment needed: Autoclave
Anaerobic workbench
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.2 | g | 0.2 | 1.47 | |
| MgCl2 | 0.1 | g | 0.1 | 1.05 | |
| NH4Cl | 0.5 | g | 0.5 | 9.348 | |
| KCl | 0.2 | g | 0.2 | 2.683 | |
| Na2CO3 | 63.6 | g | 63.6 | 600.068 | |
| NaHCO3 | 50.4 | g | 50.4 | 599.95 | |
| Na2S x 9 H2O | 0.7 | g | 0.7 | 2.914 | |
| Yeast extract | 0.2 | g | 0.2 | - | |
| Sucrose | 5.0 | g | 5 | 14.607 | |
| Modified Wolin's mineral solution | 1.0 | ml | - | - | |
| Modified Wolin's mineral solution | 10.0 | ml | - | - | |
| Resazurin | 1.0 | mg | 1.0e-3 | 0.004 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Final pH 9.5-10.0 | |||||
| 2 Prepare the medium without adding the vitamins, sucrose, yeast extract, NH4Cl, Na2CO3, and NaHCO3 using anaerobic conditions, under nitrogen. If the medium has been boiled to remove oxygen add the NH4Cl, Na2CO3, and NaHCO3 after the medium had cooled. Dispense into tubes stopper with rubber stoppers (serum tubes or bottles, or screw capped tubes). Autoclave, and to the cooled medium add the vitamins (filter sterilised under N2), sucrose, yeast extract, and Na2S x 9 H2O from anaerobic, sterile stock solutions. | |||||
Media with this solution
| 931 | ALCALIPHILIC AMPHIBACILLUS STRAINS MEDIUM |