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DSMZ

DSMZ 399 . ARCHAEOGLOBUS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 399 #

ml
Equipment needed: Autoclave Gassing station Serum bottle
Compound Amount Unit Conc. [g/L] Conc. [mM]
KCl 0.34 g 0.336 4.507
MgCl2 x 6 H2O 4.00 g 3.953 19.442
MgSO4 x 7 H2O 3.45 g 3.409 13.831
NH4Cl 0.25 g 0.247 4.618
CaCl2 x 2 H2O 0.14 g 0.138 0.941
K2HPO4 0.14 g 0.138 0.794
NaCl 18.00 g 17.787 304.356
Yeast extract
(OXOID) 		0.50 g 0.494 -
Na-L-lactate 1.50 g 1.482 -
Fe(NH4)2(SO4)2 x 7 H2O
(0.1% w/v) 		2.00 ml 0.002 0.005
Modified Wolin's mineral solution 10.00 ml - -
Sodium resazurin
(0.1% w/v) 		0.50 ml 4.9e-4 0.002
NaHCO3 3.00 g 2.964 35.288
Na2S x 9 H2O 0.50 g 0.494 2.057
Distilled water 1000.00 ml - -
1 Dissolve ingredients except bicarbonate and sulfide, then sparge medium with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add bicarbonate and adjust pH to 6.9, then dispense medium under 80% H2 and 20% CO2 gas atmosphere into serum vials (e.g., 20 ml per 100 ml bottle), seal and autoclave. Before use reduce the medium with sodium sulfide from a sterile anoxic stock solution prepared under 100% N2 gas.
2 After inoculation, add 2 bar overpressure of sterile 80% H2 and 20% CO2 gas mixture.

Modified Wolin's mineral solution #

1000
Solution occurs in 166 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.40 mg 4.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Metadata

Last modified: 17.10.22
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 6.9
Equipment needed: Autoclave, Gassing station, Serum bottle
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Gas composition

  • 80 % H2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 3.953 19.442
NaHCO3 2.964 35.288
Na-L-lactate 1.482 -
Na2S x 9 H2O 0.494 2.057
Yeast extract 0.494 -
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Cultivation metadata from 3 strains BacDive

phyla 1 3 strains
oxygen anaerobe 3 strains
temperature hyperthermophilic 2 strains
temperature thermophilic 1 strain