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924b. METHANOSARCINA MB MEDIUM (1 G/L NACL)

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.00 g 0.98 16.775
2 1.00 g 0.98 4.822
3 0.50 g 0.49 6.575
4 0.40 g 0.392 2.667
5 0.40 g 0.392 2.252
6 1.00 g 0.98 18.328
7 Trace element solution 10.00 ml - -
8
(OXOID)
2.00 g 1.961 -
9
(BD BBL)
2.00 g 1.961 -
10
(0.1% w/v)
0.50 ml 4.9e-4 0.002
11 4.00 g 3.922 46.682
12 6.80 g 6.667 98.029
13 1.60 g 1.569 19.122
14 Vitamin solution 10.00 ml - -
15 0.25 g 0.245 1.395
16 0.25 g 0.245 1.02
17 1000.00 ml 978.634 54322.4
18 , might need strain adjustment
(20% w/v)
2.00 g/l - -
19 , might need strain adjustment 4.00 ml/l - -
Instructions:
  1. Dissolve ingredients (except bicarbonate, formate, acetate vitamins, and reducing agents), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add solid bicarbonate, adjust pH to 6.8 - 7.0, dispense medium under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add formate, acetate, vitamins (sterilized by filtration), cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. The pH of the complete medium should be 7.2.
Solution occurs in 192 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.50 g 1.5 7.848
2 3.00 g 3 12.172
3 0.50 g 0.5 2.958
4 1.00 g 1 17.111
5 0.10 g 0.1 0.36
6 0.18 g 0.18 0.64
7 0.10 g 0.1 0.68
8 0.18 g 0.18 0.626
9 0.01 g 0.01 0.04
10 0.02 g 0.02 0.042
11 0.01 g 0.01 0.162
12 0.01 g 0.01 0.041
13 0.03 g 0.03 0.126
14 0.30 mg 3.0e-4 0.001
15 0.40 mg 4.0e-4 0.001
16 1000.00 ml 998.207 55408.8
Instructions:
  1. First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 3
Strains with modifications: 3

Gas composition

  • 100 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
Sodium formate 6.667 98.029
NaHCO3 3.922 46.682
Trypticase peptone 1.961 -
Yeast extract 1.961 -
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Cultivation metadata from 3 strains BacDive

phyla 1 3 strains
oxygen anaerobe 3 strains
temperature mesophilic 3 strains

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