DSMZ 1234 . ACIDITHIOBACILLUS FERRIVORANS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2SO4 1.40 g 1.386 9.759
(NH4)2SO4 3.00 g 2.97 22.478
KCl 0.10 g 0.099 1.328
MgSO4 x 7 H2O 0.50 g 0.495 2.009
K2HPO4 0.05 g 0.05 0.284
Ca(NO3)2 x 4 H2O 0.02 g 0.02 0.084
(NH4)2Ni(SO4)2 x 6 H2O 0.02 g 0.02 0.05
Modified Wolin's mineral solution 10.00 ml - -
K2S4O6 0.80 g 0.792 -
FeSO4 x 7 H2O 0.03 g 0.03 0.107
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except tetrathionate and ferrous sulfate), adjust pH to 2.5 with H2SO4 and autoclave. After cooling, add tetrathionate from a 100 mM stock solution and FeSO4 from a 10 mM stock solution (prepared in water adjusted to pH 2.0 with H2SO4). Both solutions should be freshly prepared and sterilized by filtration.
Solution occurs in 166 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.40 mg 4.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Metadata

Last modified: 13.10.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: n.d.
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 3
Strains with modifications: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
(NH4)2SO4 2.97 22.478
Na2SO4 1.386 9.759
K2S4O6 0.792 -
KCl 0.099 1.328
K2HPO4 0.05 0.284
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Cultivation metadata from 3 strains BacDive

pH 2 - 4 1 strain
pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 1 3 strains
oxygen aerobe 1 strain
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