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738. BPHD Medium

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.00 g 0.98 18.328
2 0.42 g 0.412 2.313
3 0.18 g 0.176 -
4 0.10 g 0.098 0.482
5 0.10 g 0.098 0.667
6 0.10 g 0.098 1.315
7 0.04 g 0.039 0.141
8 Vitamin solution 10.00 ml - -
9 Trace element solution 10.00 ml - -
10 , either 1.00 mM - 1
11 , or
(e.g.: 20 ml/l of a 4.7 g/l phenol -solution)
1.00 mM 0.094 1
12 1000.00 ml 978.634 54322.4
Instructions:
  1. Adjust pH to 6.5. Add either cresol (#10) or phenol (#11) after autoclaving.
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 192 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.50 g 1.5 7.848
2 3.00 g 3 12.172
3 0.50 g 0.5 2.958
4 1.00 g 1 17.111
5 0.10 g 0.1 0.36
6 0.18 g 0.18 0.64
7 0.10 g 0.1 0.68
8 0.18 g 0.18 0.626
9 0.01 g 0.01 0.04
10 0.02 g 0.02 0.042
11 0.01 g 0.01 0.162
12 0.01 g 0.01 0.041
13 0.03 g 0.03 0.126
14 0.30 mg 3.0e-4 0.001
15 0.40 mg 4.0e-4 0.001
16 1000.00 ml 998.207 55408.8
Instructions:
  1. First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.5

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
NH4Cl 0.98 18.328
Na2HPO4 x 2 H2O 0.412 2.313
Na2H2PO4 x H2O 0.176 -
CaCl2 x 2 H2O 0.099 0.674
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature thermophilic 1 strain

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