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Main sol. 738

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Internal Solution-ID: S1511

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 1.00 g 1 18.695
Na2HPO4 x 2 H2O 0.42 g 0.42 2.959
Na2HPO4 x H2O 0.18 g 0.18 1.268
MgCl2 x 6 H2O 0.10 g 0.1 0.492
CaCl2 x 2 H2O 0.10 g 0.1 0.68
KCl 0.10 g 0.1 1.341
FeSO4 x 7 H2O 0.04 g 0.04 0.144
Wolin's vitamin solution 10.00 ml - -
Modified Wolin's mineral solution 10.00 ml - -
Distilled water 1000.00 ml - -
Cresol OR Phenol 1.00 mM - 1
1 Dissolve phosphates separately, Adjust pH to 6.5. Add cresol/phenol and vitamin solution after autoclaving.
2 (e.g.: 20 ml/l of a 4.7 g/l phenol-solution)
738 BPHD Medium