DSMZ 1570 . THIOGRANUM MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 30.00 g 30 513.347
CaCl2 x 2 H2O 0.15 g 0.15 1.02
MgCl2 x 6 H2O 3.05 g 3.05 15.002
KCl 0.33 g 0.33 4.427
(NH4)2SO4 0.66 g 0.66 4.995
Modified Wolin's mineral solution 10.00 ml - -
Phosphate buffer 5.00 ml - -
Na2S2O3 x 5 H2O 1.50 g 1.5 6.044
Na2CO3 1.00 g 1 9.435
Wolin's vitamin solution 10.00 ml - -
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except phosphate buffer, thiosulfate, carbonate and vitamins) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials up to a volume of 20% and autoclave. Add phosphate buffer, thiosulfate, carbonate, and vitamin solution to the autoclaved medium from sterile anoxic stock solutions prepared under 100% N2 gas. Phosphate buffer, stock solutions of vitamins and thiosulfate are sterilized by filtration and stored under N2, whereas the solution of carbonate is prepared under 80% N2 and 20% CO2 gas mixture and autoclaved. Adjust pH of the complete medium to 6.7, if necessary. After inoculation pressurize vessels to 0.5 bar overpressure with sterile 80% N2 and 20% CO2 gas mixture and add sterile air in an amount that is equivalent to a volume of 20% of the headspace.
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 119 g 119 874.453
K2HPO4 21 g 21 120.568
Distilled water 1000 ml - -
Solution occurs in 170 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.40 mg 4.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
Solution occurs in 357 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 11.05.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.7
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
Edit in Medium builder

Gas composition

  • 71 % N2
  • 18 % CO2
  • 12 % Air

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 3.05 15.002
Na2S2O3 x 5 H2O 1.5 6.044
Na2CO3 1 9.435
(NH4)2SO4 0.66 4.995
KH2PO4 0.595 4.372
Show more

Cultivation metadata from 2 strains BacDive

halophily slightly halophilic 1 strain
pH 6 - 8 1 strain
phyla 1 2 strains
nutrition chemolithoautotroph 1 strain
oxygen facultative anaerobe 1 strain
Show more