DSMZ 165 . ACETIVIBRIO CELLULOLYTICUS MEDIUM

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Culture medium recipe

Vol.
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Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Mineral solution 1 75.00 ml - -
Mineral solution 2 75.00 ml - -
FeSO4 x 7 H2O 0.02 g 0.02 0.072
Wolin's vitamin solution 10.00 ml - -
1 Adjust pH to 7.6. Gas atmosphere: 80% N2 + 20% CO2. Final pH after autoclaving is 7.2. Cellobiose is filter-sterilized separately, flushed and stored under nitrogen gas until needed. Cysteine and sodium sulfide are each autoclaved separately in concentrated solutions under nitrogen gas.
Modified Wolin's mineral solution 10.00 ml - -
Cellobiose OR Cellulose 3.00 g 3 8.764
Resazurin 1.00 mg 1.0e-3 0.004
NaHCO3 2.00 g 2 23.808
L-Cysteine HCl x H2O 0.25 g 0.25 1.423
Na2S x 9 H2O 0.25 g 0.25 1.041
Distilled water 830.00 ml - -
2 Adjust pH to 7.6. Gas atmosphere: 80% N2 + 20% CO2. Final pH after autoclaving is 7.2. Either use Cellobiose (filter-sterilized separately, flushed and stored under nitrogen gas until needed) or Cellulose (MN 300, Whatman CF II, Kleenex tissue paper, or HCl treated cotton). Cysteine and sodium sulfide are each autoclaved separately in concentrated solutions under nitrogen gas.
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 3.9 g 3.9 22.391
Distilled water 1000.0 ml - -
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 2.40 g 2.4 17.636
(NH4)2SO4 6.00 g 6 45.406
NaCl 0.59 g 0.59 10.096
MgSO4 x 7 H2O 1.20 g 1.2 4.869
CaCl2 x 2 H2O 0.72 g 0.72 4.897
Distilled water 1000.00 ml - -
Solution occurs in 357 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -
Solution occurs in 170 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.40 mg 4.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Metadata

Last modified: 24.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Autoclave, Filter
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Cellobiose 3 8.764
Cellulose 3 -
NaHCO3 2 23.808
(NH4)2SO4 0.45 3.405
K2HPO4 0.293 1.679
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain