286 .
CLOSTRIDIUM AMINOBUTYRICUM MEDIUM
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Equipment needed:
Autoclave
Gassing station
Hungate tubes
Final gas composition:
100 % N2
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Final pH:
7.2
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
KH2PO4 | 1.30 | g | ||
|
|
K2HPO4 | 7.00 | g | ||
|
|
MgCl2 x 6 H2O | 0.20 | g | ||
|
|
FeCl3 x 6 H2O | 0.01 | g | ||
|
|
CaCl2 x 2 H2O | 0.01 | g | ||
|
|
Na2MoO4 x 2 H2O
(0.1% w/v) |
1.00 | ml | ||
|
|
Yeast extract | 3.00 | g | ||
|
|
gamma-Aminobutyric acid | 5.00 | g | ||
|
|
Agar | 1.50 | g | ||
|
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | ||
|
|
Na2S x 9 H2O | 0.30 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Dissolve ingredients (except sulfide), adjust pH to 7.2, bring medium to the boil, then cool to room temperature under 100% N2 gas. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add sulfide from a sterile anoxic stock solution prepared under 100% N2 gas atmosphere. | |||||