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293. PROPIONIGENIUM MODESTUM MEDIUM (MARINE)

Culture medium recipe

Vol.
×
Equipment needed: Filter Hungate tubes Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.20 g 0.2 1.467
2 0.25 g 0.25 4.664
3 20.00 g 19.96 341.534
4 3.00 g 2.994 14.727
5 0.50 g 0.499 6.693
6 0.15 g 0.15 1.018
7 Trace element solution SL-10 1.00 ml - -
8
(0.1% w/v)
0.50 ml 5.0e-4 0.002
9 2.50 g 2.495 29.7
10 3.25 g 3.244 20.015
11 0.36 g 0.359 1.496
12 1000.00 ml 996.215 55298.2
13
optional
0.10 % 1 -
14 , might need strain adjustment 0.68 g/l - -
15 , might need strain adjustment 0.90 g/l - -
16
optional
2.00 g/l 2 -
17
(molecular weight 106 - 20000)
optional
1.00 g/l 1 -
18 , might need strain adjustment 1.00 g/l - -
19 Vitamin solution , might need strain adjustment 10.00 ml/l - -
20 , might need strain adjustment 1.00 g/l - -
Instructions:
  1. Dissolve ingredients except bicarbonate, succinate and sulfide, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under the same gas atmosphere into anoxic Hungate-type tubes and autoclave. Add succinate (sterilized by filtration) and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of complete medium to 7.2 - 7.5.
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.5
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 9
Strains with modifications: 9

Gas composition

  • 20 % CO2
  • 80 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 997.201 55352.9
NaCl 19.96 341.534
Na2-succinate 3.244 20.015
MgCl2 x 6 H2O 2.994 14.727
NaHCO3 2.495 29.7
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Cultivation metadata from 9 strains BacDive

phyla 2 8 strains
oxygen anaerobe 8 strains
temperature mesophilic 8 strains

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