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31. ALKALINE NUTRIENT AGAR

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5 g 5 -
2 3 g 3 -
3
optional
15 g 15 -
4 1000 ml 998.207 55408.8
5 , recommended for sporulation of Bacillus strains
optional
10 mg 0.01 0.059
6 1M Na-sesquicarbonate solution 100 ml - -
Instructions:
  1. Same as medium 1. After sterilization add sterile 1 M Na-sesquicarbonate solution (1 ml in 10 ml) to achieve a pH of 9.7.
  2. For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation.
Solution occurs in 1 other media (first defined in medium 31 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 4.2 g 42 499.961
2 5.3 g 53 500.057
3 100.0 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 9.7

Associated strains

All strains for this medium: 151
Strains with modifications: 69

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 1098.03 60949.7
Agar
optional
15 -
Na2CO3 5.3 50.006
Peptone 5 -
NaHCO3 4.2 49.996
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Cultivation metadata from 151 strains BacDive

halophily moderate 9 strains
halophily not 6 strains
halophily slightly 4 strains
pH 8 - 10 10 strains
pH 6 - 8 10 strains
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Similar media

1. NUTRIENT AGAR 85 %
1a. REACTIVATION WITH LIQUID MEDIUM 1 85 %
302. NUTRIENT BROTH WITH 10% HORSE SERUM 82 %
238. STREPTOMYCIN NUTRIENT AGAR 82 %
118. DAP-NUTRIENT AGAR 82 %

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