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DSMZ

DSMZ 335 . EUBACTERIUM OXIDOREDUCENS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 335 #

ml
Equipment needed: Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 892 ml - -
Solution B 100 ml - -
Solution C 10 ml - -
Solution D 1 ml - -
1 Prepare solution A under 80% N2 and 20% CO2 gas mixture, distribute 8.9 ml per Hungate-type tube under same gas atmosphere and autoclave. Filter sterilize NaHCO3 solution and gas for 20 min with 80% N2 and 20% CO2 gas mixture. Autoclave cysteine and sulfide under 100% N2 gas. Complete medium by adding the solutions B, C, and D with a syringe to solution A. Adjust final pH of medium to 7.0 - 7.5.

Solution A #

892
Compound Amount Unit Conc. [g/L] Conc. [mM]
Mineral solution 50 ml - -
Trace element solution SL-10 1 ml - -
Yeast extract 2 g 2.242 -
Crotonic acid 5 g 5.605 65.111
Seven vitamins solution 1 ml - -
Resazurin 1 mg 0.001 0.005
Distilled water 840 ml - -
1 Adjust pH to 6.9 before autoclaving.

Solution B #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaHCO3 5 g 50 595.188
Distilled water 100 ml - -

Solution C #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
L-Cysteine HCl x H2O 0.24 g 24 136.646
Distilled water 10.00 ml - -

Solution D #

1
Equipment needed: Hungate tubes Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2S x 9 H2O 78 mg 78 324.754
Distilled water 1 ml - -

Mineral solution #

1000
Solution occurs in 4 other media (first defined in Medium 335).
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 10.0 g 10 73.484
MgCl2 x 6 H2O 6.6 g 6.6 32.464
NaCl 8.0 g 8 136.893
NH4Cl 8.0 g 8 149.561
CaCl2 x 2 H2O 1.0 g 1 6.802
Distilled water 1000.0 ml - -

Trace element solution SL-10 #

1000
Solution occurs in 266 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Seven vitamins solution #

1000
Solution occurs in 78 other media (first defined in Medium 503).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 100 mg 0.1 0.074
p-Aminobenzoic acid 80 mg 0.08 0.583
D-(+)-biotin 20 mg 0.02 0.082
Nicotinic acid 200 mg 0.2 1.625
Calcium pantothenate 100 mg 0.1 0.21
Pyridoxine hydrochloride 300 mg 0.3 1.459
Thiamine-HCl x 2 H2O 200 mg 0.2 0.536
Distilled water 1000 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0 - 7.5
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Associated strains

All strains for this medium: 1
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaHCO3 4.985 59.341
Crotonic acid 4.985 57.905
Yeast extract 1.994 -
KH2PO4 0.499 3.663
NH4Cl 0.399 7.456
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain