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335. EUBACTERIUM OXIDOREDUCENS MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Hungate tubes Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Solution A 890 ml - -
2 Solution B 100 ml - -
3 Solution C 10 ml - -
4 Solution D 1 ml - -
Instructions:
  1. Prepare solution A under 80% N2 and 20% CO2 gas mixture, distribute 8.9 ml per Hungate-type tube under same gas atmosphere and autoclave. Filter sterilize NaHCO3 solution and gas for 20 min with 80% N2 and 20% CO2 gas mixture. Autoclave cysteine and sulfide under 100% N2 gas. Complete medium by adding the solutions B, C, and D with a syringe to solution A. Adjust final pH of medium to 7.0 - 7.5.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Mineral solution 50 ml - -
2 Trace element solution SL-10 1 ml - -
3 2 g 2.242 -
4 5 g 5.605 65.111
5 Seven vitamins solution 1 ml - -
6 1 mg 0.001 0.005
7 840 ml 940.016 52178.7
Instructions:
  1. Adjust pH to 6.9 before autoclaving.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5 g 50 595.191
2 100 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.24 g 24 136.646
2 10.00 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 78 mg 78 324.754
2 1 ml 998.207 55408.8
Solution occurs in 3 other media (first defined in medium 335 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 10.0 g 10 73.484
2 6.6 g 6.6 32.464
3 8.0 g 8 136.887
4 8.0 g 8 149.557
5 1.0 g 1 6.802
6 1000.0 ml 998.207 55408.8
Solution occurs in 86 other media (first defined in medium 503 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 100 mg 0.1 0.063
2 80 mg 0.08 0.583
3 20 mg 0.02 0.082
4 200 mg 0.2 1.625
5 100 mg 0.1 0.21
6 300 mg 0.3 1.459
7 200 mg 0.2 0.536
8 1000 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.5
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.197 55408.3
NaHCO3 4.995 59.46
Crotonic acid 4.984 57.891
Yeast extract 1.994 -
KH2PO4 0.498 3.662
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain

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