Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
This strain uses the standard medium without modifications.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Beef extract
(Difco) |
3.00 | g | 3 | - | |
Trypticase
(BBL) |
9.00 | g | 9 | - | |
Yeast extract | 11.00 | g | 11 | - | |
NaCl | 2.00 | g | 2 | 34.223 | |
Na2HPO4 | 0.40 | g | 0.4 | 2.818 | |
Na2CO3 | 0.25 | g | 0.25 | 2.359 | |
Haemin | 5.00 | mg | 0.005 | 0.008 | |
Na-formate | 2.00 | g | 2 | 29.409 | |
Na-fumarate | 3.00 | g | 3 | 18.746 | |
Resazurin | 1.00 | mg | 1.0e-3 | 0.004 | |
Distilled water | 1000.00 | ml | - | - | |
1 Adjust pH to 7.5. Prepare the medium anaerobically under 100% N2. | |||||
2 Autoclave solutions of haemin (dissolved in 0.1 ml 1N NaOH, then diluted with water to 10 ml and neutralized), formate, and fumarate separately under nitrogen. |
Last modified: | 07.09.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 7.5 |
Equipment needed: | Autoclave, Gassing station, Hungate tubes |
Edit in Medium builder |
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
All strains for this medium: | 2 |
No modifications necessary for this strain.
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Yeast extract | 11 | - |
Trypticase | 9 | - |
Na-fumarate | 3 | 18.746 |
Beef extract | 3 | - |
NaCl | 2 | 34.223 |
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