Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Trypticase
(BBL) |
17 | g | 17 | - | |
Yeast extract | 3 | g | 3 | - | |
Glucose | 3 | g | 3 | 16.652 | |
NaCl | 3 | g | 3 | 51.335 | |
KNO3 | 3 | g | 3 | 29.673 | |
Haemin | 3 | mg | 0.003 | 0.005 | |
Distilled water | 1000 | ml | - | - | |
1 Adjust pH to 7.0. | |||||
2 Sterilize glucose separately. Incubate in oxygen-free, 5 - 10% CO2 containing atmosphere. |
Last modified: | 07.09.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 7.0 |
Equipment needed: | Autoclave, Gassing station, Hungate tubes |
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Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
All strains for this medium: | 4 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Trypticase | 17 | - |
NaCl | 3 | 51.335 |
KNO3 | 3 | 29.673 |
Glucose | 3 | 16.652 |
Yeast extract | 3 | - |
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