350 .
CELLULOMONAS FERMENTANS MEDIUM
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Equipment needed:
Autoclave
Gassing station
Hungate tubes
Final gas composition:
100 % N2
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Final pH:
7.4
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
K2HPO4 | 2.21 | g | ||
|
|
KH2PO4 | 1.50 | g | ||
|
|
(NH4)2SO4 | 1.30 | g | ||
|
|
MgCl2 x 6 H2O | 0.10 | g | ||
|
|
CaCl2 x 2 H2O | 0.02 | g | ||
|
|
FeSO4 x 7 H2O | 1.25 | mg | ||
|
|
Yeast extract | 5.00 | g | ||
|
|
Cellobiose
(cellulose: MN 300) |
5.00 | g | ||
|
|
NaHCO3 | 0.80 | g | ||
|
|
Resazurin | 1.00 | mg | ||
|
|
L-Cysteine HCl x H2O | 0.50 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Adjust pH to 7.4 with 8 M NaOH. | |||||
| 2 Prepare the medium anaerobically under 100% nitrogen. Cellobiose, NaHCO3, and cysteine are sterilized separately by autoclaving under N2. | |||||