388 .
DICTYOGLOMUS MEDIUM
What is this page?
Equipment needed:
Autoclave
Filter
Gassing station
Hungate tubes
Final gas composition:
100 % N2
read more
Final pH:
7.2
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
KH2PO4 | 1.50 | g | ||
|
|
Na2HPO4 x 12 H2O | 4.20 | g | ||
|
|
NH4Cl | 0.50 | g | ||
|
|
MgCl2 x 6 H2O | 0.38 | g | ||
|
|
CaCl2 x 2 H2O | 0.06 | g | ||
|
|
Fe(NH4)2(SO4)2 x 6 H2O | 0.04 | g | ||
|
|
CoCl2 x 6 H2O
(0.1% w/v) |
2.90 | ml | ||
|
|
Na2MoO4 x 2 H2O
(0.1% w/v) |
2.40 | ml | ||
|
|
Na2SeO3 x 5 H2O
(0.01% w/v) |
1.70 | ml | ||
|
|
MnCl2 x 4 H2O
(0.1% w/v) |
2.00 | ml | ||
|
|
ZnSO4
(0.1% w/v) |
2.80 | ml | ||
|
|
Yeast extract | 2.00 | g | ||
|
|
Polypeptone | 2.00 | g | ||
|
|
Starch
(soluble) |
5.00 | g | ||
|
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | ||
|
|
Na2CO3 | 1.00 | g | ||
|
|
L-Cysteine HCl x H2O | 1.00 | g | ||
|
|
Wolin's vitamin solution (10x) | 1.00 | ml | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Dissolve ingredients (except carbonate, cysteine and vitamins), bring medium to the boil, then cool to room temperature under 100% N2 gas atmosphere. Add and dissolve carbonate and cysteine, adjust pH to 7.2, then dispense under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add vitamins from an anoxic stock solution prepared under 100% N2 gas and sterilized by filtration. Adjust pH of complete medium to 7.2, if necessary. | |||||
Wolin's vitamin solution (10x) #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 20 | mg |
|
|
Folic acid | 20 | mg |
|
|
Pyridoxine hydrochloride | 100 | mg |
|
|
Thiamine HCl | 50 | mg |
|
|
Riboflavin | 50 | mg |
|
|
Nicotinic acid | 50 | mg |
|
|
Calcium D-(+)-pantothenate | 50 | mg |
|
|
Vitamin B12 | 1 | mg |
|
|
p-Aminobenzoic acid | 50 | mg |
|
|
(DL)-alpha-Lipoic acid | 50 | mg |
|
|
Distilled water | 1000 | ml |