DSMZ 429e . e. COLUMBIA BLOOD AGAR 10%

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Equipment needed:

Final pH: n.d.

Select a strain (optional):

ml
Compound Amount Unit
Columbia agar base 1000 ml
Horse blood 10 %
1 Columbia agar base supplemented with 10% horse blood. Rehydrate pellets of freeze-dried ampoules in liquid medium 545, 215 or 1 (see below). For rehydration of Helicobacter and Campylobacter spp., use medium 215 (brain heart infusion).

545. TRYPTONE SOYA BROTH (TSB) #

1000
Compound Amount Unit
Casein peptone 17.0 g
Soy peptone 3.0 g
D(+)-Glucose 2.5 g
NaCl 5.0 g
K2HPO4 2.5 g
Distilled water 1000.0 ml
1 Adjust pH to 7.3.
2 The same as Caso Bouillon (Merck or Oxoid, Germany).

215. BHI MEDIUM #

1000
Compound Amount Unit
Brain heart infusion
(Difco)
37 g
Distilled water 1000 ml

1. NUTRIENT BROTH #

1000
Compound Amount Unit
Peptone 5 g
Meat extract 3 g
Distilled water 1000 ml
1 Adjust pH to 7.0.