432
432 .
HALOBACTEROIDES ACETOETHYLICUS MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Medium for Halanaerobium acetethylicum DSM 3532
This strain uses the standard medium without modifications.
Growth conditions: 30 °C
Main sol. 432 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Medium 144 | 1000 | ml | - | - | |
| NaCl | 100 | g/l | 100 | 1711.16 | |
| 1 Use medium no. 144 supplemented with 100 g/l of NaCl. | |||||
| 2 Sterilize glucose, yeast extract and sodium sulfide separately (autoclave under nitrogen atmosphere). | |||||
Solution occurs in 1
other media (first defined in Medium 144).
Equipment needed: Autoclave
Hungate tubes
Filter
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 0.90 | g | 0.9 | 16.826 | |
| NaCl | 0.90 | g | 0.9 | 15.4 | |
| MgCl2 x 6 H2O | 0.40 | g | 0.4 | 1.968 | |
| KH2PO4 | 0.75 | g | 0.75 | 5.511 | |
| K2HPO4 | 1.50 | g | 1.5 | 8.612 | |
| Trace element solution | 9.00 | ml | - | - | |
|
FeSO4 x 7 H2O
(0.1% w/v in 0.1 N H2SO4) |
3.00 | ml | 0.003 | 0.011 | |
| Yeast extract | 3.00 | g | 3 | - | |
|
Trypticase peptone
(BD BBL) |
10.00 | g | 10 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | - | |
| Wolin's vitamin solution | 5.00 | ml | - | - | |
| D-Glucose | 5.00 | g | 5 | 27.753 | |
| Na2S x 9 H2O | 1.00 | g | 1 | 4.164 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except vitamins, glucose and sulfide), sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add glucose, vitamins (sterilized by filtration) and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Adjust pH of complete medium to 7.2 - 7.4. | |||||
Solution occurs in 53
other media (first defined in Medium 141).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.0 | mg | 0.002 | 0.008 |
| Folic acid | 2.0 | mg | 0.002 | 0.005 |
| Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
| Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 |
| Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
| Calcium D-(+)-pantothenate | 5.0 | mg | 0.005 | 0.021 |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
| p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
| (DL)-alpha-Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 15.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Equipment needed: | Autoclave, Filter, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Halanaerobium acetethylicum DSM 3532
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 100 | 1711.16 |
| Trypticase peptone | 10 | - |
| D-Glucose | 5 | 27.753 |
| Yeast extract | 3 | - |
| K2HPO4 | 1.5 | 8.612 |
| Show more | ||
