435
435 .
KDM-2 MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 435 #
ml
Equipment needed: Autoclave
Water bath
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Peptone | 10.0 | g | 10 | - | |
| Yeast extract | 0.5 | g | 0.5 | - | |
| L-Cysteine HCl x H2O | 1.0 | g | 1 | 5.694 | |
| Agar | 15.0 | g | 15 | 44.599 | |
| Distilled water | 800.0 | ml | - | - | |
| Activated charcoal | 2.0 | g/l | 2 | - | |
| Fetal bovine serum | 200.0 | ml | - | - | |
| 1 Adjust pH to 6.5. | |||||
| 2 Dissolve peptone, yeast extract and cysteine in 800 ml of distilled water. Adjust pH to 6.5 with NaOH. Add agar and dissolve by heating. | |||||
| 3 Add 2 g/l activated charcoal to the agar base solution before autoclaving. After autoclaving, cool to 45°C and add sterilized serum. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 6.5 |
| Equipment needed: | Autoclave, Water bath |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 15 | 44.599 |
| Peptone | 10 | - |
| Activated charcoal | 2 | - |
| L-Cysteine HCl x H2O | 1 | 5.694 |
| Yeast extract | 0.5 | - |
| Show more | ||
