456 .
KPL MEDIUM
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Equipment needed:
Autoclave
Filter
Final pH:
5.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Lactic acid whey | 1000 | ml | ||
|
|
White table wine | 140 | ml | ||
|
|
Glucose | 10 | g | ||
|
|
Galactose | 10 | g | ||
|
|
Tween 80 | 1 | ml | ||
|
|
Agar | 15 | g | ||
| 1 Adjust pH to 5.5 | |||||
Lactic acid whey #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Skim milk | 10 | % | ||
| 1 Adjust 10% skim milk to pH 5.5 with lactic acid and heat at 100°C for 30 minutes. Remove precipitate by filtration. For preparing broth medium, adjust filtrate to pH 7.0 with 2 N NaOH and heat at 100°C for 30 minutes. Remove precipitate by filtration. Adjust filtrate to pH 5.5 with 2 N HCl. | |||||
White table wine #
200
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
White wine | 200 | ml | ||
| 1 Filter-sterilize and add to autoclaved basal medium. | |||||