469 .
YM-CATALASE MEDIUM
What is this page?
Equipment needed:
Autoclave
Filter
Final pH:
7.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Yeast extract | 5.0 | g | ||
|
|
Malt extract | 5.0 | g | ||
|
|
K2HPO4 | 5.7 | g | ||
|
|
MgSO4 x 7 H2O | 0.2 | g | ||
|
|
NH4H2PO4 | 1.2 | g | ||
|
|
Catalase
(Sigma C-10) |
60.0 | mg | ||
|
|
Distilled water | 980.0 | ml | ||
| 1 Adjust pH to 7.0. Add catalase and MgSO4 to 10 ml of distilled water each, and sterilize separately by filtration. Cool down autoclaved main solution to 50°C, and aseptically add catalase and MgSO4 solution. | |||||