470 .
PSEUDOMONAS HALOPHILA MEDIUM
What is this page?
Equipment needed:
Autoclave
Filter
Final pH:
7.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Solution A | 900 | ml | ||
|
|
Solution B | 100 | ml | ||
| 1 Vitamin solution is filter-sterilized and added after autoclaving. Solution A and B are autoclaved separately and combined after cooling. | |||||
| 2 Adjust pH to 7.0 with 6 M NaOH or HCl. | |||||
Solution A #
900
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
NaCl | 46.8 | g |
|
|
MgSO4 x 7 H2O | 39.4 | g |
|
|
NH4Cl | 1.0 | g |
|
|
Glycerol | 5.0 | g |
|
|
Trace element solution SL-10 | 1.0 | ml |
|
|
Vitamin solution | 10.0 | ml |
|
|
Distilled water | 890.0 | ml |
Trace element solution SL-10 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
HCl
(25%) |
10.0 | ml | ||
|
|
FeCl2 x 4 H2O | 1.5 | g | ||
|
|
ZnCl2 | 70.0 | mg | ||
|
|
MnCl2 x 4 H2O | 100.0 | mg | ||
|
|
H3BO3 | 6.0 | mg | ||
|
|
CoCl2 x 6 H2O | 190.0 | mg | ||
|
|
CuCl2 x 2 H2O | 2.0 | mg | ||
|
|
NiCl2 x 6 H2O | 24.0 | mg | ||
|
|
Na2MoO4 x 2 H2O | 36.0 | mg | ||
|
|
Distilled water | 990.0 | ml | ||
| 1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. | |||||
Vitamin solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 2.00 | mg |
|
|
Folic acid | 2.00 | mg |
|
|
Pyridoxine hydrochloride | 10.00 | mg |
|
|
Thiamine-HCl x 2 H2O | 5.00 | mg |
|
|
Riboflavin | 5.00 | mg |
|
|
Nicotinic acid | 5.00 | mg |
|
|
Calcium pantothenate | 5.00 | mg |
|
|
p-Aminobenzoic acid | 1.00 | mg |
|
|
Vitamin B12 | 0.01 | mg |
|
|
Distilled water | 1000.00 | ml |