514e .
MARINE BROTH (ANAEROB)
What is this page?
Equipment needed:
Autoclave
Gassing station
Hungate tubes
Final gas composition:
100 % N2
read more
Final pH:
7.3 - 7.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Peptone
(BD Bacto) |
5.00 | g | ||
|
|
Yeast extract
(BD Bacto) |
1.00 | g | ||
|
|
NaCl | 19.45 | g | ||
|
|
MgCl2
(anhydrous) |
5.90 | g | ||
|
|
Na2SO4 | 3.24 | g | ||
|
|
KCl | 0.55 | g | ||
|
|
NaHCO3 | 0.16 | g | ||
|
|
KBr | 0.08 | g | ||
|
|
SrCl2 | 34.00 | mg | ||
|
|
H3BO3 | 22.00 | mg | ||
|
|
Na-silicate | 4.00 | mg | ||
|
|
NaF
(0.1% w/v) |
2.40 | ml | ||
|
|
(NH4)NO3
(0.1% w/v) |
1.60 | ml | ||
|
|
Na2HPO4
(0.1% w/v) |
8.00 | ml | ||
|
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | ||
|
|
L-Cysteine HCl x H2O | 0.30 | g | ||
|
|
Na2S x 9 H2O | 0.30 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Dissolve ingredients except cysteine and sulfide, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Prior to use check pH of complete medium and adjust to 7.3 - 7.5, if necessary. | |||||