514g .
HALF STRENGTH BACTO MARINE BROTH with 50% Seawater
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Equipment needed:
Final pH:
7.6
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Bacto peptone | 2.500 | g | ||
|
|
Yeast extract
(Bacto ) |
0.500 | g | ||
|
|
Fe(III) citrate | 0.050 | g | ||
|
|
NaCl | 9.725 | g | ||
|
|
MgCl2
(anhydrous) |
2.950 | g | ||
|
|
Na2SO4 | 1.620 | g | ||
|
|
CaCl2 | 0.900 | g | ||
|
|
KCl | 0.275 | g | ||
|
|
NaHCO3 | 0.080 | g | ||
|
|
KBr | 0.040 | g | ||
|
|
SrCl2 | 17.000 | mg | ||
|
|
H3BO3 | 11.000 | mg | ||
|
|
Na-silicate | 2.000 | mg | ||
|
|
NaF | 1.200 | mg | ||
|
|
(NH4)NO3 | 0.800 | mg | ||
|
|
Na2HPO4 | 4.000 | mg | ||
|
|
Sea water | 500.000 | ml | ||
|
|
Distilled water | 500.000 | ml | ||
|
|
Agar , for solid medium | 15.000 | g/l | ||
| 1 Final pH should be 7.6 ± 0.2 at 25°C. If using Difco Bacto Marine Broth use 18.7 g/l to 1 litre water. | |||||
| 2 The medium may be solidified by adding 15 g/l agar. | |||||