529 .
AMPHIBACILLUS MEDIUM
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Selected strain: Amphibacillus cookii DSM 23721
This strain uses the standard medium without modifications.
Growth conditions: facultative anaerobic
37 °C
Equipment needed:
Autoclave
Final pH:
9.7
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Glucose | 10.0 | g | ||
|
|
K2HPO4 | 1.0 | g | ||
|
|
NH4NO3 | 2.0 | g | ||
|
|
MgSO4 x 7 H2O | 0.2 | g | ||
|
|
MnSO4 x 7 H2O | 5.0 | mg | ||
|
|
FeSO4 x 7 H2O | 5.0 | mg | ||
|
|
CaCl2 x 2 H2O | 0.1 | g | ||
|
|
Yeast extract | 3.0 | g | ||
|
|
Polypeptone
(BBL) |
0.3 | g | ||
|
|
Agar | 15.0 | g | ||
|
|
Distilled water | 900.0 | ml | ||
|
|
Na-sesquicarbonate solution | 100.0 | ml | ||
| 1 After sterilization add sterile 1 M Na-sesquicarbonate solution (1 ml in 10 ml) to achieve a pH of 9.7 (see medium 31). | |||||
Na-sesquicarbonate solution #
100
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
NaHCO3 | 4.2 | g |
|
|
Na2CO3 anhydrous
(anhydrous) |
5.3 | g |
|
|
Distilled water | 100.0 | ml |