DSMZ 535b . REACTIVATION WITH LIQUID MEDIUM

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Equipment needed: Autoclave

Final pH: 7.3

Select a strain (optional):

ml
Compound Amount Unit
Medium 535 , alternative 1000 ml
545. TRYPTONE SOYA BROTH (TSB) , alternative 1000 ml
1 Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 545. Subsequent subculturing may be carried out in liquid medium 545 or on agar plates.

545. TRYPTONE SOYA BROTH (TSB) #

1000
Compound Amount Unit
Casein peptone 17.0 g
Soy peptone 3.0 g
D(+)-Glucose 2.5 g
NaCl 5.0 g
K2HPO4 2.5 g
Agar 15.0 g
Distilled water 1000.0 ml
1 Adjust pH to 7.3.
2 Medium 545 is the same as Caso Bouillon (Merck or Oxoid, Germany).

Main sol. 535 #

1000
Compound Amount Unit
Trypticase soy broth
(BBL 11768, Oxoid CM129 or Merck 5459)
30 g
Agar 20 g
Distilled water 1000 ml
1 pH 7.3
2 Autoclave at 121°C for 15 min.