546 .
LC BROTH MEDIUM
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Equipment needed:
Final pH:
7.4
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Tryptone | 10 | g | ||
|
|
Yeast extract | 5 | g | ||
|
|
NaCl | 10 | g | ||
|
|
Glucose | 1 | g | ||
|
|
Distilled water | 990 | ml | ||
|
|
CaCl2 x 2 H2O
(1 M) |
5 | ml | ||
|
|
MgSO4 x 7 H2O
(1 M) |
5 | ml | ||
| 1 Adjust pH to 7.4. | |||||
| 2 Sterilize at 121°C for 15 min. Cool to 50°C and add 5 ml 1 M CaCl2 x 2 H2O and 5 ml 1 M MgSO4 x 7 H2O as sterile solutions. | |||||