547
547 .
ISP MEDIUM 4
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 547 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Solution I | 500 | ml | - | - | |
| Solution II | 501 | ml | - | - | |
| Agar | 20 | g | 19.98 | 59.406 | |
| 1 The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Starch
(soluble) |
10 | g | 20 | - | |
| Distilled water | 500 | ml | - | - | |
| 1 Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| CaCO3 | 2 | g | 3.992 | 39.884 |
|
K2HPO4
(anhydrous) |
1 | g | 1.996 | 11.46 |
| MgSO4 x 7 H2O | 1 | g | 1.996 | 8.098 |
| NaCl | 1 | g | 1.996 | 34.155 |
| (NH4)2SO4 | 2 | g | 3.992 | 30.211 |
| Distilled water | 500 | ml | - | - |
| Trace salt solution | 1 | ml | - | - |
Solution occurs in 6
other media (first defined in Medium 547).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| FeSO4 x 7 H2O | 0.1 | g | 1 | 3.597 |
| MnCl2 x 4 H2O | 0.1 | g | 1 | 5.053 |
| ZnSO4 x 7 H2O | 0.1 | g | 1 | 3.478 |
| Distilled water | 100.0 | ml | - | - |
Metadata
| Last modified: | 19.09.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.2 |
| Edit in Medium builder | |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 19.98 | 59.406 |
| Starch | 9.99 | - |
| CaCO3 | 1.998 | 19.962 |
| (NH4)2SO4 | 1.998 | 15.12 |
| NaCl | 0.999 | 17.095 |
| Show more | ||
