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547. ISP MEDIUM 4

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Solution I x ml - -
2 Solution II 501 ml - -
3 20 g 39.92 -
Instructions:
  1. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(DIFCO)
10 g 20 -
2 500 ml 998.207 55408.8
Instructions:
  1. Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2 g 3.992 39.886
2
(anhydrous)
1 g 1.996 11.46
3 1 g 1.996 8.098
4 1 g 1.996 34.153
5 2 g 3.992 30.211
6 500 ml 996.215 55298.2
7 Trace salt solution 1 ml - -
Instructions:
  1. The pH should be between 7.0 and 7.4. Do not adjust if it is within this range.
Solution occurs in 2 other media (first defined in medium 547 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.1 g 1 3.597
2 0.1 g 1 5.053
3 0.1 g 1 3.478
4 100.0 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.4

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
Agar 39.92 -
CaCO3 3.992 39.886
(NH4)2SO4 3.992 30.211
NaCl 1.996 34.153
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