55 .
FRUCTOSE MINERAL MEDIUM
What is this page?
Equipment needed:
Autoclave
Filter
Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Solution A | 500.0 | ml | ||
|
|
Solution B | 300.0 | ml | ||
|
|
Solution C | 200.0 | ml | ||
|
|
Agar , if required | 1.5 | % | ||
| 1 Autoclave solutions A and B separately, filter sterilize solution C. Add 1.5% agar if necessary. | |||||
Solution A #
500
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Na2HPO4 x 12 H2O | 9.0 | g |
|
|
KH2PO4 | 1.5 | g |
|
|
NH4Cl | 1.0 | g |
|
|
MgSO4 x 7 H2O | 0.2 | g |
|
|
Trace element solution SL-6 | 0.3 | ml |
|
|
Distilled water | 500.0 | ml |
Solution B #
300
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
CaCl2 x 2 H2O | 0.01 | g |
|
|
Ferric ammonium citrate | 5.00 | mg |
|
|
Distilled water | 300.00 | ml |
Trace element solution SL-6 #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
ZnSO4 x 7 H2O | 0.10 | g |
|
|
MnCl2 x 4 H2O | 0.03 | g |
|
|
H3BO3 | 0.30 | g |
|
|
CoCl2 x 6 H2O | 0.20 | g |
|
|
CuCl2 x 2 H2O | 0.01 | g |
|
|
NiCl2 x 6 H2O | 0.02 | g |
|
|
Na2MoO4 x 2 H2O | 0.03 | g |
|
|
Distilled water | 1000.00 | ml |