DSMZ 589 . HALOBACTERIUM LACUSPROFUNDII MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 181.0 g 181 3097.19
MgCl2 x 6 H2O 75.0 g 75 368.908
MgSO4 x 7 H2O 7.4 g 7.4 30.023
KCl 7.4 g 7.4 99.262
CaCl2 x 2 H2O 1.0 g 1 6.802
Yeast extract 1.0 g 1 -
Sodium succinate 10.0 g 10 61.709
Vitamin solution 1.0 ml - -
water (distilled or tapwater) 1000.0 ml - -
Agar , for solid medium 15.0 g/l 15 44.599
1 pH 7.4
2 Filter-sterilize vitamin solution and add to the medium when it has cooled to about 55 - 60°C. Add agar at a concentration of 15 g/l for solid media.
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 10 mg 0.1 0.409
Vitamin B12 10 mg 0.1 0.074
Thiamine-HCl x 2 H2O 10 mg 0.1 0.268
Distilled water 100 ml - -

Metadata

Last modified: 30.01.24
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 7.4
Equipment needed: Filter
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Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 181 3097.19
MgCl2 x 6 H2O 75 368.908
Agar 15 44.599
Sodium succinate 10 61.709
KCl 7.4 99.262
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Cultivation metadata from 2 strains BacDive

phyla 1 2 strains
temperature mesophilic 2 strains