616 .
ALKALITHERMOBACTER MEDIUM
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Selected strain: Alkalithermobacter paradoxus DSM 7308
This strain uses the standard medium without modifications.
Growth conditions: anaerobic
55 °C
Equipment needed:
Autoclave
Gassing station
Hungate tubes
Final gas composition:
100 % N2
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Final pH:
10.0
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ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Na2HPO4 x 2 H2O | 0.36 | g | ||
|
|
KCl | 0.08 | g | ||
|
|
Yeast extract
(OXOID) |
5.00 | g | ||
|
|
Tryptone
(BD Bacto) |
10.00 | g | ||
|
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | ||
|
|
Na2CO3 | 5.30 | g | ||
|
|
D-Glucose | 3.00 | g | ||
|
|
L-Cysteine HCl x H2O | 0.20 | g | ||
|
|
Na2S x 9 H2O | 0.20 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Dissolve ingredients (except carbonate, glucose, cysteine and sulfide) and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add and dissolve carbonate, dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Before use add glucose, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and adjust pH to 10 by adding sterile anoxic 3 N NaOH. | |||||