DSMZ 621 . PYGV AGAR

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Culture medium recipe

Vol.
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Selected strain: Geodermatophilus sp. DSM 1114

This strain uses the standard medium without modifications.

Compound Amount Unit Conc. [g/L] Conc. [mM]
Mineral salt solution
("Hutner/Cohen-Bazire")
20.00 ml - -
Peptone
(Bacto)
0.25 g 0.25 -
Yeast extract
(Bacto)
0.25 g 0.25 -
Agar
(Bacto)
15.00 g 15 44.599
Distilled water 965.00 ml - -
1 Sterilize 20 min./121°C. After cooling to 60°C add to the medium:
Glucose
(2.5%, sterile-filtered)
10.00 ml 0.25 1.388
Vitamin solution (double conc.) 5.00 ml - -
2 Adjust pH to 7.5 (the medium is only weakly buffered; one needs approx. 10 drops/l medium of 6 N KOH).
Solution occurs in 3 other media (first defined in Medium 621).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid
(NTA)
10.00 g 10.526 55.071
MgSO4 x 7 H2O 29.70 g 31.263 126.841
CaCl2 x 2 H2O 3.34 g 3.516 23.915
Na2MoO4 x 2 H2O 12.67 mg 0.013 0.055
FeSO4 x 7 H2O 99.00 mg 0.104 0.375
Metall salt sol. 44
(see medium 590)
50.00 ml - -
Distilled water 900.00 ml - -
1 Dissolve NTA first by neutralizing with KOH, then add other salts. Adjust pH to 7.2 with KOH or H2SO4. Adjust volume to 1000.0 ml with distilled water.
Solution occurs in 6 other media (first defined in Medium 621).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 4.0 mg 0.004 0.016
Folic acid 4.0 mg 0.004 0.009
Pyridoxine hydrochloride 20.0 mg 0.02 0.097
Riboflavine 10.0 mg 0.01 0.027
Thiamine-HCl x 2 H2O 10.0 mg 0.01 0.027
Nicotinamide 10.0 mg 0.01 0.082
D-Calcium pantothenate 10.0 mg 0.01 -
Vitamin B12 0.2 mg 2.0e-4 1.5e-4
p-Aminobenzoic acid 10.0 mg 0.01 0.073
Distilled water 1000.0 ml - -
1 Store in the dark and cold (5°C).

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.5
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Associated strains

All strains for this medium: 38
Strains with modifications: 11
Geodermatophilus sp. DSM 1114

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
MgSO4 x 7 H2O 0.625 2.537
Glucose 0.25 1.388
Peptone 0.25 -
Yeast extract 0.25 -
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Cultivation metadata from 38 strains BacDive

pH 6 - 8 4 strains
pH 8 - 10 3 strains
pH 4 - 6 2 strains
phyla 7 37 strains
oxygen aerobe 5 strains
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