Mineral salt solution
Internal Solution-ID: S1331
This solution is used in 4 media and was first defined in medium 621.
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| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Nitrilotriacetic acid
(NTA) |
10.00 | g | 10.526 | 55.071 | |
| MgSO4 x 7 H2O | 29.70 | g | 31.263 | 126.841 | |
| CaCl2 x 2 H2O | 3.34 | g | 3.516 | 23.915 | |
| Na2MoO4 x 2 H2O | 12.67 | mg | 0.013 | 0.055 | |
| FeSO4 x 7 H2O | 99.00 | mg | 0.104 | 0.375 | |
|
Metall salt sol. 44
(see medium 590) |
50.00 | ml | - | - | |
| Distilled water | 900.00 | ml | - | - | |
| 1 Dissolve NTA first by neutralizing with KOH, then add other salts. Adjust pH to 7.2 with KOH or H2SO4. Adjust volume to 1000.0 ml with distilled water. | |||||