632
632 .
NMS MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 632 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Methanol | 1.000 | ml | 0.792 | 24.718 | |
| MgSO4 x 7 H2O | 1.000 | g | 1 | 4.057 | |
| CaCl2 x 6 H2O | 0.200 | g | 0.2 | 0.913 | |
| Fe(III)NH4-EDTA | 4.000 | mg | 0.004 | - | |
| KNO3 | 1.000 | g | 1 | 9.891 | |
| Trace element solution | 0.500 | ml | - | - | |
| KH2PO4 | 0.272 | g | 0.272 | 1.999 | |
| Na2HPO4 x 12 H2O | 0.717 | g | 0.717 | 5.051 | |
|
Agar
(e.g. Oxoid L28, purified ) |
12.500 | g | 12.5 | 37.166 | |
| Distilled water | 1000.000 | ml | - | - | |
| 1 Adjust pH to 6.8. Autoclave at 121°C for 15 min. Methanol is added from a sterile stock solution. | |||||
Solution occurs in 1
other media (first defined in Medium 632).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Na2-EDTA | 500 | mg | 0.5 | 1.487 |
| FeSO4 x 7 H2O | 200 | mg | 0.2 | 0.719 |
| ZnSO4 x 7 H2O | 10 | mg | 0.01 | 0.035 |
| MnCl2 x 4 H2O | 3 | mg | 0.003 | 0.015 |
| H3BO3 | 30 | mg | 0.03 | 0.485 |
| CoCl2 x 6 H2O | 20 | mg | 0.02 | 0.154 |
| CaCl2 x 2 H2O | 1 | mg | 1.0e-3 | 0.007 |
| NiCl2 x 6 H2O | 2 | mg | 0.002 | 0.015 |
| Na2MoO4 x 2 H2O | 3 | mg | 0.003 | 0.012 |
| Distilled water | 1000 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | 6.8 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 12.5 | 37.166 |
| KNO3 | 1 | 9.891 |
| MgSO4 x 7 H2O | 1 | 4.057 |
| Methanol | 0.792 | 24.718 |
| Na2HPO4 x 12 H2O | 0.717 | 5.051 |
| Show more | ||
