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DSMZ

DSMZ 639 . MEDIUM WITH THIAZOLE

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 639 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 100 ml - -
Solution B 5 ml - -
Solution C 5 ml - -
1 Autoclave solutions A, B and C separately and combine after cooling 100 ml solution A, 5 ml solution B and 5 ml solution C.

Solution A #

90
Compound Amount Unit Conc. [g/L] Conc. [mM]
Benzothiazole 30 mg 0.333 2.466
NaOH
(10 %) 		90 ml 9 225.017
1 Dissolve 30.0 mg benzothiazole in 90 ml 10% NaOH, bring pH to 7.0 with concentrated HCl.

Solution B #

50
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 300 mg 6 44.09
Distilled water 50 ml - -
1 Dissolve 300 mg KH2PO4 in 50 ml distilled water.

Solution C #

50
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
CaCl2 x 2 H2O 6 mg 0.12 0.816
MgSO4 x 7 H2O 50 mg 1 4.057
FeCl3 10 mg 0.2 1.233
Distilled water 50 ml - -

Metadata

Last modified: 23.11.21
Source: DSMZ
Medium type: Defined medium
Final pH: n.d.
Equipment needed: Autoclave
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaOH 8.182 204.561
Benzothiazole 0.303 2.242
KH2PO4 0.273 2.004
MgSO4 x 7 H2O 0.045 0.184
FeCl3 0.009 0.056
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