Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
Solution A | 980 | ml | ||
|
Solution B | 10 | ml | ||
|
Solution C | 10 | ml | ||
1 Dissolve ingredients of solution A, bring to the boil, then cool to room temperature while sparging with 100% N2 gas. Add solutions B and C, adjust pH to 7.8 with NaOH, and distribute under 100% N2 gas into anoxic Hungate-type tubes. During distribution continuously swirl the medium to keep the grey precipitate suspended. Autoclave 15 min at 121°C. Adjust pH of the complete medium to 6.8 - 7.0, if necessary. |
Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
K2HPO4 | 0.5 | g | ||
|
NH4Cl | 1.0 | g | ||
|
Na2SO4 | 1.0 | g | ||
|
CaCl2 x 2 H2O | 0.1 | g | ||
|
MgSO4 x 7 H2O | 2.0 | g | ||
|
Na-DL-lactate | 2.0 | g | ||
|
Yeast extract | 1.0 | g | ||
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | ||
|
Sea water
(filtered and aged) |
980.0 | ml | ||
1 Alternatively to filtered aged sea water bottled water from Biomaris GmbH (Bremen, Germany) can be used. |
Compound | Amount | Unit | |
---|---|---|---|
|
Na-thioglycolate | 0.1 | g |
|
Ascorbic acid | 0.1 | g |
|
Distilled water | 10.0 | ml |