655 .
PYE MEDIUM
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Equipment needed:
Final pH:
6.8
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
K2HPO4 | 1.00 | g | ||
|
|
MgSO4 x 7 H2O | 0.20 | g | ||
|
|
CaCl2 x 2 H2O | 0.02 | g | ||
|
|
Na2S2O3 x 5 H2O | 0.20 | g | ||
|
|
Na-pyruvate | 2.20 | g | ||
|
|
Yeast extract
(Difco) |
4.00 | g | ||
|
|
(NH4)2SO4 | 1.00 | g | ||
|
|
Trace element solution SL-6 | 1.00 | ml | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Adjust pH to 6.8. | |||||
| 2 Boil the medium for few minutes. Fill in tubes with rubber stoppers under nitrogen gas. Sterilize at 121°C for 15 min. | |||||
Trace element solution SL-6 #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
ZnSO4 x 7 H2O | 0.10 | g |
|
|
MnCl2 x 4 H2O | 0.03 | g |
|
|
H3BO3 | 0.30 | g |
|
|
CoCl2 x 6 H2O | 0.20 | g |
|
|
CuCl2 x 2 H2O | 0.01 | g |
|
|
NiCl2 x 6 H2O | 0.02 | g |
|
|
Na2MoO4 x 2 H2O | 0.03 | g |
|
|
Distilled water | 1000.00 | ml |