659 .
M17 MEDIUM (modified)
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Equipment needed:
Filter
Final pH:
7.1 - 7.2
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Casein peptone | 5.00 | g | ||
|
|
Soy peptone | 5.00 | g | ||
|
|
Meat peptone | 5.00 | g | ||
|
|
Yeast extract | 2.50 | g | ||
|
|
Ascorbic acid | 0.50 | g | ||
|
|
MgSO4 x 7 H2O | 0.25 | g | ||
|
|
Na2-ß-glycerolphosphate | 9.50 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 After autoclaving add a lactose solution (sterilized by filtration) up to a concentration of 8.0 g/l, and 1.2 ml 1M CaCl2 x 2 H2O. Adjust pH to 7.15 ± 0.05. | |||||
|
|
Lactose
(sterilized by filtration) |
8.00 | g/l | ||
|
|
CaCl2 x 2 H2O | 1.20 | ml | ||