674 .
MEDIUM FOR ACIDURIC, THERMOPHILIC BACILLUS STRAINS
What is this page?
Equipment needed:
Autoclave
Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Solution A | 500 | ml | ||
|
|
Solution B | 500 | ml | ||
| 1 Solutions A and B are sterilized separately. After cooling to approximately 45°C solution A is poured into solution B. Mix thoroughly. | |||||
Solution A #
500
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
(NH4)2SO4 | 0.20 | g | ||
|
|
MgSO4 x 7 H2O | 0.50 | g | ||
|
|
CaCl2 x 2 H2O | 0.25 | g | ||
|
|
KH2PO4 | 3.00 | g | ||
|
|
Yeast extract | 1.00 | g | ||
|
|
Tryptone
(Bacto) |
1.00 | g | ||
|
|
Glucose | 1.00 | g | ||
|
|
Starch | 1.00 | g | ||
|
|
Distilled water | 500.00 | ml | ||
| 1 Adjust pH to 4.3. | |||||