687 .
COLUMBIA BLOOD AGAR
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Equipment needed:
Autoclave
Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Columbia Blood Agar Base | 44 | g | ||
|
|
Deionized water | 1000 | ml | ||
|
|
Defibrinated Blood
(sterile) |
5 | % | ||
| 1 Suspend 44 g Columbia Blood Agar Base in 1l deionized water. Heat to boiling to dissolve completely. | |||||
| 2 Sterilize in autoclave for 15 min at 121°C. | |||||
| 3 Cool to 45°C. Add 5% sterile defibrinated blood and mix well. | |||||
Columbia Blood Agar Base #
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Bacto Panton
(Peptone) |
10 | g |
|
|
Bacto Bitone
(Peptone) |
10 | g |
|
|
Tryptic Digest of beef heart | 3 | g |
|
|
Corn starch | 1 | g |
|
|
NaCl | 5 | g |
|
|
Agar | 15 | g |