68 .
HALOTHIOBACILLUS NEAPOLITANUS MEDIUM
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Equipment needed:
Autoclave
Final pH:
6.6 - 7.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
KH2PO4 | 4.0 | g | ||
|
|
K2HPO4 | 4.0 | g | ||
|
|
MgSO4 x 7 H2O | 0.8 | g | ||
|
|
NH4Cl | 0.4 | g | ||
|
|
Trace element solution (Vishniac & Santer, 1957) | 5.0 | ml | ||
|
|
Bromocresol purple
(saturated aqueous solution) |
0.5 | ml | ||
|
|
Agar
, for solid medium
(Bacto) optional |
15.0 | g | ||
|
|
Na2S2O3 x 5 H2O | 10.0 | g | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Dissolve ingredients except thiosulfate, dispense into suitable cultivation vessels and autoclave. Add thiosulfate to the autoclaved medium from a sterile stock solution. Adjust pH of complete medium to 6.6 - 7.0. | |||||
Trace element solution (Vishniac & Santer, 1957) #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Na2-EDTA | 50.00 | g | ||
|
|
ZnSO4 x 7 H2O | 22.00 | g | ||
|
|
CaCl2 x 2 H2O | 5.54 | g | ||
|
|
MnCl2 x 4 H2O | 5.06 | g | ||
|
|
FeSO4 x 7 H2O | 5.00 | g | ||
|
|
(NH4)6Mo7O24 x 4 H2O | 1.10 | g | ||
|
|
CuSO4 x 5 H2O | 1.57 | g | ||
|
|
CoCl2 x 6 H2O | 1.61 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Dissolve EDTA in distilled water, adjust pH to 7 using 2 N KOH, then add remaining compounds. Adjust pH of final solution to 6.0 with KOH. | |||||