807 .
HALOANAEROBIUM ALCALIPHILUM MEDIUM
What is this page?
Equipment needed:
Autoclave
Final pH:
7.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
MgSO4 x 7 H2O | 17.00 | g | ||
|
|
NaCl | 100.00 | g | ||
|
|
Solution A | 50.00 | ml | ||
|
|
Solution B | 50.00 | ml | ||
|
|
Trace element solution SL-10 | 10.00 | ml | ||
|
|
Resazurin | 1.00 | mg | ||
|
|
Yeast extract | 10.00 | g | ||
|
|
Trypticase | 10.00 | g | ||
|
|
Glucose | 5.00 | g | ||
|
|
Na2S x 9 H2O | 0.25 | g | ||
|
|
NaHCO3 | 4.10 | g | ||
|
|
L-Cysteine HCl x H2O | 0.50 | g | ||
|
|
Distilled water | 890.00 | ml | ||
| 1 pH 7.0 | |||||
| 2 Prepare the medium without yeast extract, glucose, NaHCO3, cysteine hydrochloride and Na2S x 9 H2O. Boil and then cool under a gas atmosphere of N2:CO2 (80:20 v/v) and add the NaHCO3 and cysteine hydrochloride. Autoclave the medium and add from sterile stock solutions yeast extract, trypticase, glucose, and Na2S x 9 H2O. The final pH should be 7.0. | |||||
Solution B #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
KH2PO4 | 6.00 | g |
|
|
(NH4)2SO4 | 6.00 | g |
|
|
NaCl | 12.00 | g |
|
|
MgSO4 x 7 H2O | 2.60 | g |
|
|
NH4Cl | 2.50 | g |
|
|
CaCl2 x 2 H2O | 0.28 | g |
|
|
K2HPO4 | 0.28 | g |
Trace element solution SL-10 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
HCl
(25%) |
10.0 | ml | ||
|
|
FeCl2 x 4 H2O | 1.5 | g | ||
|
|
ZnCl2 | 70.0 | mg | ||
|
|
MnCl2 x 4 H2O | 100.0 | mg | ||
|
|
H3BO3 | 6.0 | mg | ||
|
|
CoCl2 x 6 H2O | 190.0 | mg | ||
|
|
CuCl2 x 2 H2O | 2.0 | mg | ||
|
|
NiCl2 x 6 H2O | 24.0 | mg | ||
|
|
Na2MoO4 x 2 H2O | 36.0 | mg | ||
|
|
Distilled water | 990.0 | ml | ||
| 1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. | |||||