811
811 .
ACTIVATED CARBON MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 811 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2HPO4 x 12 H2O | 9.0 | g | 9 | 63.399 | |
| KH2PO4 | 1.5 | g | 1.5 | 11.023 | |
| NH4Cl | 1.5 | g | 1.5 | 28.043 | |
| MgSO4 x 7 H2O | 0.2 | g | 0.2 | 0.811 | |
| CaCl2 x 2 H2O | 20.0 | mg | 0.02 | 0.136 | |
| Fe(NH4)citrate | 1.2 | mg | 0.001 | - | |
| Trace element solution TS2 | 1.0 | ml | - | - | |
| Activated carbon | 5.0 | g | 5 | - | |
| Agar | 15.0 | g | 15 | 44.599 | |
| Double distilled water | 1000.0 | ml | - | - | |
| 1 Befor autoclaving add trace element solution to the medium. Dissolve first all ingredients then add activated carbon. Finally autoclave. | |||||
| 2 pH 7.5 | |||||
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| ZnSO4 x 7 H2O | 100 | mg | 0.1 | 0.348 |
| MnCl2 x 4 H2O | 30 | mg | 0.03 | 0.152 |
| H3BO3 | 300 | mg | 0.3 | 4.851 |
| CoCl2 x 6 H2O | 200 | mg | 0.2 | 1.54 |
| CuCl2 x 2 H2O | 10 | mg | 0.01 | 0.059 |
| NiCl2 x 6 H2O | 20 | mg | 0.02 | 0.154 |
| Na2MoO4 x 2 H2O | 900 | mg | 0.9 | 3.72 |
| Na2SeO3 | 20 | mg | 0.02 | 0.116 |
| Distilled water | 1000 | ml | - | - |
Metadata
| Last modified: | 20.09.22 |
| Source: | DSMZ |
| Medium type: | Defined medium |
| Final pH: | 7.5 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |