MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ

DSMZ 816 . SPOROTOMACULUM MEDIUM

What is this page?
Equipment needed: Autoclave Gassing station Hungate tubes
Final gas composition: 80 % N2 20 % CO2 read more
Final pH: 7.6
Select a strain (optional):

ml
Compound Amount Unit
Solution A 920 ml
Solution B 1 ml
Solution C 1 ml
Solution D 50 ml
Solution E 10 ml
Solution F 5 ml
Solution G 5 ml
Solution H 10 ml
1 Sparge solution A with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Solutions B, C, F, G, and H are autoclaved separately under 100% N2 gas. Solution D is autoclaved under 80% N2 and 20% CO2 gas atmosphere and autoclaved. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. To complete the medium appropriate amounts of solutions B to H are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be checked and adjusted to 7.6 with a sterile anoxic stock solution of Na2CO3 (5% w/v), if necessary.

Solution A #

920
Compound Amount Unit
KH2PO4 0.20 g
NH4Cl 0.30 g
KCl 0.50 g
NaCl 1.00 g
CaCl2 x 2 H2O 0.15 g
MgCl2 x 6 H2O 0.40 g
Sodium resazurin
(0.1% w/v) 		0.50 ml
Distilled water 920.00 ml

Solution B #

1
Compound Amount Unit
Trace element solution SL-10 1 ml

Solution C #

1
Compound Amount Unit
Selenite-tungstate solution 1 ml

Solution D #

50
Compound Amount Unit
Na2CO3 2.5 g
Distilled water 50.0 ml

Solution E #

10
Compound Amount Unit
3-Hydroxybenzoic acid 2.8 g
Distilled water 10.0 ml
1 Neutralized with NaOH.

Solution F #

5
Compound Amount Unit
Yeast extract 0.5 g
Distilled water 5.0 ml

Solution G #

5
Compound Amount Unit
Trypticase
(BD BBL) 		0.5 g
Distilled water 5.0 ml

Solution H #

10
Compound Amount Unit
Na2S x 9 H2O 0.3 g
Distilled water 10.0 ml

Trace element solution SL-10 #

1000
Compound Amount Unit
HCl
(25%) 		10.0 ml
FeCl2 x 4 H2O 1.5 g
ZnCl2 70.0 mg
MnCl2 x 4 H2O 100.0 mg
H3BO3 6.0 mg
CoCl2 x 6 H2O 190.0 mg
CuCl2 x 2 H2O 2.0 mg
NiCl2 x 6 H2O 24.0 mg
Na2MoO4 x 2 H2O 36.0 mg
Distilled water 990.0 ml
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Selenite-tungstate solution #

1000
Compound Amount Unit
NaOH 0.5 g
Na2SeO3 x 5 H2O 3.0 mg
Na2WO4 x 2 H2O 4.0 mg
Distilled water 1000.0 ml