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DSMZ

DSMZ 842 . HALOANAEROBACTER SALINARIUS MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
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Medium for Orenia salinaria DSM 13466

This strain uses the standard medium without modifications.

Growth conditions: 3-7 days anaerobic 30 °C

Main sol. 842 #

ml
Equipment needed: Anaerobic workbench Hungate tubes Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 150.00 g 150 2566.74
MgCl2 x 6 H2O 15.00 g 15 73.782
KCl 3.00 g 3 40.241
NH4Cl 0.50 g 0.5 9.348
KH2PO4 0.35 g 0.35 2.572
CaCl2 x 2 H2O 0.05 g 0.05 0.34
Yeast extract 0.10 g 0.1 -
Na2S x 9 H2O 0.50 g 0.5 2.082
NaHCO3 2.00 g 2 23.808
Glucose 1.20 g 1.2 6.661
Resazurin 1.00 mg 1.0e-3 0.004
Trace element solution SL-12 B 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
Vitamin solution 7 1.00 ml - -
Distilled water 997.00 ml - -
1 Final pH 7.2 - 7.4
2 Prepare the medium without yeast extract, Na2S x 9 H2O, NaHCO3, glucose and vitamin solution (prepared as anaerobic, sterile stock solutions). Boil the medium and cool under anaerobic gas mixture of N2:CO2 (80:20). Dispense medium into Hungate tubes or serum bottles using the same gas mixture. Autoclave. Add the remaining components to the medium once it has cooled and check the final pH.

Selenite-tungstate solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2SeO3 x 5 H2O 6.0 mg 0.006 0.023
Na2WO4 x 2 H2O 8.0 mg 0.008 0.024
NaOH 0.4 g 0.4 10.001
Distilled water 1000.0 ml - -

Vitamin solution 7 #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 10 mg 0.01 0.041
p-Aminobenzoic acid 50 mg 0.05 0.365
Vitamin B12 50 mg 0.05 0.037
Thiamine 100 mg 0.1 0.377
Distilled water 1000 ml - -

Trace element solution SL-12 B #

1000
Solution occurs in 4 other media (first defined in Medium 28).
Equipment needed: Ice bath Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 3.0 g 3 8.923
FeSO4 x 7 H2O 1.1 g 1.1 3.957
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
MnCl2 x 2 H2O 50.0 mg 0.05 -
ZnCl2 42.0 mg 0.042 0.308
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 18.0 mg 0.018 0.074
H3BO3 300.0 mg 0.3 4.851
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
Distilled water 1000.0 ml - -
1 Adjust pH to 6.0.

Metadata

Last modified: 24.03.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2 - 7.4
Equipment needed: Anaerobic workbench, Autoclave, Filter, Hungate tubes, Ice bath
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 2
Orenia salinaria DSM 13466

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 150 2566.74
MgCl2 x 6 H2O 15 73.782
KCl 3 40.241
NaHCO3 2 23.808
Glucose 1.2 6.661
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Cultivation metadata from 2 strains BacDive

phyla 1 2 strains
oxygen anaerobe 2 strains
temperature mesophilic 2 strains