857
857 .
BACTO MIDDLEBROOK 7H9 BROTH
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 857 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Ammonium Sulfate | 0.50 | g | 0.5 | 3.784 | |
| Monopotassium Phosphate | 1.00 | g | 1 | 7.348 | |
| Disodium Phosphate | 2.50 | g | 2.5 | 17.611 | |
| Sodium citrate | 0.10 | g | 0.1 | 0.387 | |
| Magnesium sulfate | 0.05 | g | 0.05 | 0.415 | |
| Calcium Chloride | 0.50 | mg | 5.0e-4 | 0.003 | |
| Zinc Sulfate | 1.00 | mg | 1.0e-3 | 0.006 | |
| Copper Sulfate | 1.00 | mg | 1.0e-3 | 0.006 | |
| L-Glutamic acid | 0.50 | g | 0.5 | 3.398 | |
| Ferric ammonium citrate | 0.04 | g | 0.04 | - | |
| Pyridoxine | 1.00 | mg | 1.0e-3 | 0.006 | |
| Biotin | 0.50 | mg | 5.0e-4 | 0.002 | |
| Distilled water | 1000.00 | ml | - | - | |
| ADC Enrichment | 100.00 | ml | - | - | |
| 1 Solution g/liter (final solution) 4.7 g/l. Final pH at 25°C 6.6 ± 0.2. | |||||
| 2 To prepare one liter of final medium, suspend the amount of dehydrated powder indicated under FORMULAE-Solution (and on the package label) in 900 ml distilled and deionized water containing 2 ml glycerol and heat to boiling to dissolve completely. Sterilize in the autoclave for 10 or 15 min at 15 Ibs pressure (121°C), per label copy. Cool to 50-55°C. | |||||
| 3 Aseptically add 100 ml of the appropriate ADC Enrichment and mix throughly. | |||||
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
|
Bovine albumin
(albumin fraction V) |
5.0 | g | 50 | - |
| Dextrose | 2.0 | g | 20 | 100.923 |
|
Catalase
(Beef) |
0.3 | mg | 0.003 | - |
| Distilled water | 100.0 | ml | - | - |
Metadata
| Last modified: | 20.09.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 6.4 - 6.8 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |