864 .
HALOTHIOBACILLUS MEDIUM
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Equipment needed:
Autoclave
Final pH:
6.5 - 7.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaCl | 29.00 | g | ||
|
|
(NH4)2SO4 | 1.00 | g | ||
|
|
MgSO4 x 7 H2O | 1.50 | g | ||
|
|
CaCl2 x 2 H2O | 0.42 | g | ||
|
|
KCl | 0.70 | g | ||
|
|
Trace element solution SL-11 | 1.00 | ml | ||
|
|
Bromothymol blue
(0.1% w/v) |
4.00 | ml | ||
|
|
K2HPO4 | 0.50 | g | ||
|
|
Na2S2O3 x 5 H2O | 5.00 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Dissolve ingredients except hydrogenphosphate and thiosulfate, adjust pH to 6.5, then dispense medium into culture vessels and autoclave. Add hydrogenphosphate and thiosulfate from sterile stock solutions. Adjust pH of complete medium to 6.5 - 7.0. | |||||
Trace element solution SL-11 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Na2-EDTA x 2 H2O | 5.2 | g | ||
|
|
FeCl2 x 4 H2O | 1.5 | g | ||
|
|
ZnCl2 | 70.0 | mg | ||
|
|
MnCl2 x 4 H2O | 100.0 | mg | ||
|
|
H3BO3 | 6.0 | mg | ||
|
|
CoCl2 x 6 H2O | 190.0 | mg | ||
|
|
CuCl2 x 2 H2O | 2.0 | mg | ||
|
|
NiCl2 x 6 H2O | 24.0 | mg | ||
|
|
Na2MoO4 x 2 H2O | 36.0 | mg | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Dissolve EDTA in 800 ml distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add other compounds. Finally adjust pH to 6.0 and bring volume to 1000 ml. | |||||