DSMZ 86a . MODIFIED CASTENHOLZ MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid
(Titriplex I)
100.00 mg 0.1 0.523
CaSO4 x 2 H2O 60.00 mg 0.06 0.348
MgSO4 x 7 H2O 100.00 mg 0.1 0.406
NaCl 8.00 mg 0.008 0.137
KNO3 103.00 mg 0.103 1.019
NaNO3 689.00 mg 0.689 8.106
Na2HPO4 x 2 H2O 140.00 mg 0.14 0.986
FeCl3 x 6 H2O 0.47 mg 4.7e-4 0.002
MnSO4 x H2O 2.20 mg 0.002 0.013
ZnSO4 x 7 H2O 0.50 mg 5.0e-4 0.002
H3BO3 0.50 mg 5.0e-4 0.008
CuSO4 x 5 H2O 25.00 µg 2.5e-5 1.0e-4
Na2MoO4 x 2 H2O 25.00 µg 2.5e-5 1.0e-4
CoCl2 x 6 H2O 46.00 µg 4.6e-5 3.5e-4
Peptone 3.00 g 3 -
Yeast extract 1.00 g 1 -
Monosodium glutamate 1.00 g 1 5.913
Distilled water 1000.00 ml - -
Agar , if required 2.25 % 25 74.332
1 Adjust pH to 7.8 with NaOH.
2 Solid media is prepared by the addition of 2 - 2.5% agar.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.8
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Associated strains

All strains for this medium: 3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 25 74.332
Peptone 3 -
Monosodium glutamate 1 5.913
Yeast extract 1 -
NaNO3 0.689 8.106
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Cultivation metadata from 3 strains BacDive

phyla 1 3 strains
temperature thermophilic 3 strains