886 .
HELIORESTIS MEDIUM
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Equipment needed:
Filter
Final pH:
9.0 - 9.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
KH2PO4 | 0.500 | g | ||
|
|
NH4Cl | 0.500 | g | ||
|
|
NaCl | 0.500 | g | ||
|
|
MgCl2 x 6 H2O | 0.600 | g | ||
|
|
CaCl2 | 0.100 | g | ||
|
|
Na2CO3 | 2.500 | g | ||
|
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NaHCO3 | 2.500 | g | ||
|
|
Na-acetate | 1.000 | g | ||
|
|
Yeast extract | 0.500 | g | ||
|
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Na2S x 9 H2O | 0.400 | g | ||
|
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Vitamin B12 | 20.000 | µg | ||
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Biotin | 20.000 | µg | ||
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Trace element solution SL-6 | 1.000 | ml | ||
|
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Distilled water | 1000.000 | ml | ||
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Resazurin | 0.002 | % | ||
| 1 Boil the medium for few minutes. Fill in tubes with rubber stoppers under nitrogen gas. Sterilize at 121°C for 15 min. | |||||
| 2 Prepare NaHCO3, Na2CO3 and sulfide as separate sterile stock solution, Vitamin B12 and Biotin as filter-sterilized stock solution and add to sterile medium after autoclaving. Adjust pH to 9 - 9.5 . | |||||
| 3 To control anoxic conditions, add resazurin to a final concentration of 0.002%. | |||||
Trace element solution SL-6 #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
ZnSO4 x 7 H2O | 0.10 | g |
|
|
MnCl2 x 4 H2O | 0.03 | g |
|
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H3BO3 | 0.30 | g |
|
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CoCl2 x 6 H2O | 0.20 | g |
|
|
CuCl2 x 2 H2O | 0.01 | g |
|
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NiCl2 x 6 H2O | 0.02 | g |
|
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Na2MoO4 x 2 H2O | 0.03 | g |
|
|
Distilled water | 1000.00 | ml |